2017-8-11 16:0:32025-05-24 08:37:36
description
response regulator aspartate phosphatase, dephosphorylates Spo0F-P
response regulator aspartate phosphatase, dephosphorylates [[protein|Spo0F]]-P
locus
BSU02820
BSU_02820
geneLength
1119
1122
function
control of the phosphorelay
control of the [SW|phosphorelay]
ec
outlinks
bsu
BSU02820
BSU_02820
The protein
Protein family
RAP family (according to Swiss-Prot)
[SW|RAP family] (according to UniProt)
The protein
[SW|Domains]
RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [[protein|PhrC]], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. [Pubmed|23526881]
RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain (five [SW|TPR repeat|tetratrichopeptide repeats]) that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [[protein|PhrC]], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. [Pubmed|23526881]
The protein
Paralogous protein(s)
[[this]]
Biological materials
Mutant
BKE02820 (Δ[[gene|rapJ]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTA
BKK02820 (Δ[[gene|rapJ]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGGTCTGCCTCCTTCCT, downstream forward: _UP4_TAGGAAATGGCAGAGAACTA